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      新8-羥基脫氧鳥苷(8-OHdG) ELISA試劑盒 New 8-OHdG ELISA Kit
      2021-02-07 2399 分享到:

      New 8-OHdG Check ELISA
8-oxo-dG/8-hydroxy-2'-deoxyguanosine             最暢銷的8-OHdG ELISA試劑盒

      DNA氧化生物標志物

      JaICA新的8羥基脫氧鳥苷(8-OHdG) ELISA試劑盒,適用于對尿液樣本進行氧化應激的評估。


      關于8-羥基-2'-脫氧鳥苷(8-OHdG):

      8ohdg_struct.jpg

      8-羥基-2'-脫氧鳥苷(8-OHdG),又稱為:8-羥基脫氧鳥苷;英文名稱:8-hydroxy-2'-deoxyguanosine。


      8-羥基-2-脫氧鳥苷(8-OHdG)是由羥基自由基、單線態氧和直接的光動力作用形成的DNA氧化損傷產物。8-OHdG可以在組織、血清、尿液和其它生物樣品中檢測。新的8-OHdG Check試劑盒是一種競爭性酶聯免疫吸附測定(ELISA),利用對DNA損傷高度特異性的單克隆抗體(克隆#N45.1),與RNA氧化產物(例如8-羥基鳥嘌呤和8-羥基鳥苷)沒有交叉反應。該試劑盒適用于從人和動物的尿液及其他生物樣品中檢測8-OHdG濃度。


      2(248).png

      該8羥基脫氧鳥苷(8-OHdG) ELISA試劑盒利用抗8-羥基脫氧鳥苷(8-OHdG)單克隆抗體(克隆號N45.1)對8-OHdG的高度特異性識別原理設計。在進行操作前請閱讀完整說明書或咨詢北京中昊新生(zhscience.com)。


      JaICA提供兩種類型的8-OHdG ELISA試劑盒,具有不同的檢測范圍。新的8-OHdG ELISA試劑盒適用于人或動物的尿液和血清樣品。如果需要檢測人血清、組織、細胞培養樣品的8-OHdG, 我們建議使用“高靈敏的8-羥基脫氧鳥苷ELISA試劑盒(貨號 #KOG-HS10E)”。



      技術參數:

      檢測原理:競爭法ELISA檢測(450nm);

      特異性:特異性識別8-OHdG,抗體已經過多種8-OHdG類似物的測試(guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G);

      測定范圍:0.5?200ng/mL;

      格式:96孔板(18個樣品,3個平行對照);

      應用:人和動物的尿液(對于血清和組織樣本,建議使用高靈敏的8-OHdG Check ELISA試劑盒,貨號 #KOG-HS10E);

      儲存:4 - 10°C(不能冷凍);

      保質期:12個月(應在開啟后2周內使用)。


      試劑盒組份

      WX20210207-153419.png



      References

      1)H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)

      [ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]

      2)R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)

      [ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]

      3)S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)

      [ Specifity of the antibody clone N45.1.]

      4)Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)

      [ Development and assessment of 8-OHdG Check ELISA. ]

      5)Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T, Yoshimura M, Ogawa H: Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 95(4), p514-517 (2005)

      [ Measurement of 8-OHdG in human urine. ]

      6)Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J: The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5-6),p543-549(2005)

      [ Study about exercise, antioxidant supplements and urinary 8-OHdG. ]

      7)Someya T, Kaneko K, Yamada T, Yamashiro Y: Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol 20(10),p1430-1434(2005)

      [ Effect of antioxidants to 8-OHdG in rat urine. ]

      8)Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci 250(1-2),p159-161(2006)

      [ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]


      日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 專注于衰老研究領域。提供DNA氧化損傷檢測試劑盒,并且處于該領域的領先地位。JaICA氧化應激相關產品:DNA氧化損傷檢測、脂質氧化檢測、蛋白氧化檢測、抗氧化分析,以及微量元素分析。詳情請咨詢JaICA中國區代理商:北京中昊新生科技(zhscience.com)。

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